A rice geranylgeranyl reductase is essential for chloroplast development

摘要

Chloroplasts are essential for plant photosynthesis and growth.Many genes have been identified that regulate plant chloroplast development.However,it is not known at a molecular level how these genes regulate chloroplast biogenesis.In this study,we isolated a mutant ygl2(yellow-green leaf2)that exhibited a pigment-defective phenotype.YGL2 encodes a geranylgeranyl reductase,and in mutant ygl2,there was a single base change(T1361G)located in the third exon of YGL2 that resulted in a missense mutation(L454R)in the encoded product.Transmission electron microscopy revealed that chloroplast development was impaired in the ygl2 mutant.The expression levels of plastid-encoded genes were significantly altered in the ygl2 mutant.Furthermore,in a yeast two-hybrid assay,we found that YGL2 interacted with the RNA editing factor MORF8.