Effects of Yimu Shenghuasan Preparation on the Cytochrome P450 in Endometrial Cells and Immune Function of Dairy Cows

摘要

In order to investigate the mode of action of Yimu Shenghuasan preparation in endometrial cells of dairy cows,the primary cultured endometrial cells in cows were isolated and the inflammatory models were made by lipopolysaccharide(LPS)induction.The inflammatory cells were treated with gradient concentration of herbal medicine preparation,Yimu Shenghuasan for 48 and 72 h.The expression of cytochrome P450(CYP450)was detected by Western blot.The amounts of IgG and IgA in sera were also detected in the endometritis of dairy cows.The expression level of CYP450 in the endometrial cells of dairy cow was increased gradually,and the amounts of IgG,IgA were increased significantly as compared with those in the control group.The expression level of CYP450 in the inflammatory cells was increased significantly in the treatment of 2 000 μg mL-1 of Yimu Shenghuasan after 48 h of treatment.