1B Specific LMW-GS Primers Cloned a 1D Located Gene from Wheat Cultivar Xiaoyan 22

摘要

In the present study,one unique low-molecular-weight glutenin subunit (LMW-GS) gene,LMWXY22-2 (GenBank no.FJ028810),was isolated from wheat cultivar Xiaoyan 22 (Triticum aestivum L.) by a pair of genomic specific PCR primers for 1B chromosome.Sequence analysis revealed that LMWXY22-2 was composed of 1 364 bp nucleotides,including a 317 bp promotion region and a 1 047 bp coding region which could be translated into a mature protein of 349 amino acids.In spite of a few minor mutations,the sequence of 5' untranslated region (UTR),the coding region,the deduced N-and Cterminus comparisons indicated that LMWXY22-2 belonged to the reported subunits of LMW-m type and type III group 5,respectively.Inner gene markers for 1D chromosome together with the phylogenetic analysis revealed that this gene was classified into Glu-D3,which was not in agreement with the 1B locus-specific primers for LMW genes completely.