Development of Soybean EST-SSR Markers and Their Use to Assess Genetic Diversity in the Subgenus Soja

摘要

Developing expressed sequence tag-derived SSR(EST-SSR)markers is imperative in genetic research.In this paper,we reported 37 EST-SSR markers which were developed from 286 unigenes obtained from soybean cDNA library.Among the 286 markers designed for the 4 accessions of Glycine max and 6 of its wild progenitor(G.soja)within the subgenus Soja,209 markers amplified DNA fragments,taking 73.1% and 37 markers appeared to be polymorphic,which was 12.9% of the total.The 37 loci detected a total of 142 alleles,while the PIC values varied from 0.194 to 0.794.Both the number of alleles per locus and PIC value were significantly related to the SSR motif.Six EST-SSR loci may be fixed for different alleles between G.max and G.soja since they were particularly polymorphic among the 6 G.soja accessions.A neighbor-joining tree placed the G.max accessions together as a group within the G.soja,though the average genetic distance among G.soja accessions was much higher.These new EST-SSRs markers will be useful for genetic diversity analysis,genetic mapping construction and gene discovery in Soja subgenus.