17β-Estradiol Regulates Cultured Immature Boar Sertoli Cell Proliferation via the cAMP-ERK1/2 Pathway and the Estrogen Receptor β

摘要

Estrogen plays an important role in regulating Sertoli cell number in the testis.The objective of the study was to identify whether 17β-estradiol affected the proliferation of cultured,immature boar Sertoli cells via the estrogen receptor β(ERβ) and the cAMP-extracellular signal-regulated kinase(ERK1/2) pathway.Low levels(10-10-10-8 mol L-1) of 17β-estradiol increased cell number,but high levels(10-7-10-6 mol L-1) decreased it(P 0.05).The effects of 17β-estradiol(10-9 mol L-1) peaked at the first 24 h(P 0.05).Therefore,17βestradiol mainly promotes pig Sertoli cell proliferation via ERβ to induce cAMP production and ERK activation to promote cell proliferation.