首页> 中文期刊> 《农业科学学报:英文版》 >Vegetative Compatibility and Asexual Recombination in Magnaporthe grisea from Jiangsu Province, China

Vegetative Compatibility and Asexual Recombination in Magnaporthe grisea from Jiangsu Province, China

         

摘要

Vegetative compatibility among isolates of different races in Magnaporthe grisea collected from Jiangsu Province and asexual recombination among compatible isolates by anastomosis were tested.Twenty isolates involving seven races from diseased rice plants were paired on polished rice rose bengal medium and incubated at 25℃ in darkness for 18 days. Among 173 pairings tested, solid hyphal fusion lines formed by anastomosis between 124 pairings, indicated that these isolates were vegetative compatible with each other.The result showed that most M. grisea isolates were vegetative compatible. Furthermore, 17 vegetative compatible pairings between monoconidial isolates with MBC^sipt^r marker and isolates with MBC^rIPT^s marker were selected to detect the asexual recombination between the compatible isolates of different races. The asexual recombinants with MBCrIPT~ marker were detected in single hyphal fragment progenies in thirteen of the seventeen pairings. The percentage of recombinants was about 0.6 - 11.3 %. Results showed that vegetative compaUbility was prevailing among isolates of M. grisea in Jiangsu Province in vitro. These results also suggested that asexttal recombination may be an important mechaxfism for M. grisea to maintain genetic diversity in nature.
获取原文

客服邮箱:kefu@zhangqiaokeyan.com

京公网安备:11010802029741号 ICP备案号:京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有
  • 客服微信

  • 服务号