Study of Swine Leukocyte Antigen Class 1-3(SLA-3) Gene for Inbreeding Wuzhishan Pig

摘要

To elucidate the structure of SLA-3 alleles on inbred line of Wuzhishan pig(WZSP)population,we examined the partial exon 1,completed exon 2,and partial exon 3 of SLA-3 loci using the reverse transcription-polymerase chain reaction(RTPCR)and the sequencing-based method in 32 WZSPs.According to pedigree and amplification results,PCR products of 8WZSPs were selected to clone and sequence.Nine different nucleotide sequences were obtained.After comparing the DNA and protein sequences of the WZSPs SLA-3 alleles with the published GenBank SLA sequences,it was found that the SLA-3 alleles in WZSPs were all novel,but there were very few variations among them.Comparision of SLA-3 and HLA-A protein sequences indicated that there was more sequence homology.Meanwhile,the construction of a phylogenetic tree using the nucleotide sequences of 23 SLA-3 alleles and 1 HLA-A allele represented that the WZSP population owns its unique genetics resource.In this study,the alleles of SLA-3 on WZSP group were successfully detected and analyzed,which provided the finn basis on the genotype of SLA-3 for breeding specific haplotypes WZSPs.