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《农业科学学报(英文版)》
>Transcriptional Regulation of Expression of the Maize Aldehyde Dehydrogenase 7 Gene (ZmALDH7B6) in Response to Abiotic Stresses
Transcriptional Regulation of Expression of the Maize Aldehyde Dehydrogenase 7 Gene (ZmALDH7B6) in Response to Abiotic Stresses
Aldehyde dehydrogenases (ALDHs) represent a large protein family, which includes several members that catalyze the oxidation of an aldehyde to its corresponding carboxylic acid in plants. Genes encoding members of theALDH7 subfamily have been suggested to play important roles in various stress adaptations in plants. In this study, quantitative RT-PCR analysis revealed that a maizeALDH7 subfamily member (ZmALDH7B6) was constitutively expressed in various organs, including roots, leaves, immature ears, tassels, and developing seeds. The abundance ofZmALDH7B6 mRNA transcripts in maize roots was increased by ammonium, NaCl, and mannitol treatments. To further analyze tissue-speciifc and stress-induced expression patterns, the 1.5-kb 5´-lfankingZmALDH7B6 promoter region was fused to the β-glucuronidase (GUS) reporter gene and introduced into maize plants. In roots of independent transgenic lines, there was signiifcant induction of GUS activity in response to ammonium supply, conifrming ammonium-dependent expression ofZmALDH7B6 at the transcript level. Histochemical staining showed that GUS activity driven by theZmALDH7B6 promoter was mainly localized in the vascular tissues of maize roots. These results suggested thatZmALDH7B6 is induced by multiple environmental stresses in maize roots, and may play a role in detoxifying aldehydes, particularly in vascular tissue.
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