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Mycotoxin detection-Recent trends at global level

机译:霉菌毒素检测-全球最新趋势

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摘要

Mycotoxin contamination in agro-food systems has been a serious concern globaly during the last few decades. Mycotoxins are toxic secondary metabolites produced by fungi when they grow in agro-food products and feedstuff. Several detection techniques have been developed in recent years to detect mycotoxins in the food and feed effectively. HPLC based tech-niques are very common in usage in the laboratories for the testing of mycotoxins. In recent years, immuno-based assays is widely used and have been reported at large due to its sensitivity and limited detection time. Immuno assay-based kits were developed effectively to be used in the ifelds and in storage systems to detect the mycotoxin levels. Microarray-based immunoassays developed in the recent years could simultaneously detect alfatoxin, ochratoxin, and zearalenone with the higher sensitivity. Aptamer-based assays could target the detection of ochratoxin and alfatoxins and fumonisins at high speciifcity in food products. In recent years, several assays reported for the simultaneous multiple detection of different mycotoxin was based on HPLC and LC-MS/MS. There is a need for the use of these advanced technologies in the com-mercial scale.
机译:在过去的几十年中,农业食品系统中的霉菌毒素污染一直是全球关注的严重问题。霉菌毒素是真菌在农产品和饲料中生长时产生的有毒次生代谢产物。近年来,已开发出多种检测技术来有效检测食品和饲料中的霉菌毒素。基于HPLC的技术在实验室中用于霉菌毒素测试非常普遍。近年来,基于免疫的测定法被广泛使用,并且由于其灵敏度高和检测时间有限而被大量报道。有效开发了基于免疫测定的试剂盒,可用于田间和存储系统中检测真菌毒素水平。近年来开发的基于微阵列的免疫分析方法可以同时检测出黄曲霉毒素,曲霉毒素和玉米赤霉烯酮,并且灵敏度更高。基于适体的测定可以针对食品中高特异性的曲霉毒素,黄曲霉毒素和伏马毒素进行检测。近年来,报道了几种同时检测多种霉菌毒素的测定方法是基于HPLC和LC-MS / MS的。需要在商业规模上使用这些先进技术。

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  • 来源
    《农业科学学报(英文版)》 |2015年第11期|2265-2281|共17页
  • 作者单位

    Institute of Agro-Products Processing Science and Technology, Chinese Academy of Agricultural Sciences/Key Laboratory of Agro-Products Processing, Ministry of Agriculture, Beijing 100193, P.R.China;

    Institute of Agro-Products Processing Science and Technology, Chinese Academy of Agricultural Sciences/Key Laboratory of Agro-Products Processing, Ministry of Agriculture, Beijing 100193, P.R.China;

    Institute of Agro-Products Processing Science and Technology, Chinese Academy of Agricultural Sciences/Key Laboratory of Agro-Products Processing, Ministry of Agriculture, Beijing 100193, P.R.China;

    Institute of Agro-Products Processing Science and Technology, Chinese Academy of Agricultural Sciences/Key Laboratory of Agro-Products Processing, Ministry of Agriculture, Beijing 100193, P.R.China;

    Institute of Agro-Products Processing Science and Technology, Chinese Academy of Agricultural Sciences/Key Laboratory of Agro-Products Processing, Ministry of Agriculture, Beijing 100193, P.R.China;

    Institute of Agro-Products Processing Science and Technology, Chinese Academy of Agricultural Sciences/Key Laboratory of Agro-Products Processing, Ministry of Agriculture, Beijing 100193, P.R.China;

    Institute of Agro-Products Processing Science and Technology, Chinese Academy of Agricultural Sciences/Key Laboratory of Agro-Products Processing, Ministry of Agriculture, Beijing 100193, P.R.China;

  • 收录信息 中国科学引文数据库(CSCD);
  • 原文格式 PDF
  • 正文语种 eng
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