Evaluation of selenium and carotenoid concentrations of 200 foxtail millet accessions from China and their correlations with agronomic performance

摘要

As selenium and carotenoids are essential micronutrients, the determination of their concentrations in different varieties is important in the breeding of foxtail milet (Setaria italicaL. P.Beauv.). To identify selenium- and carotenoid-enriched foxtail milet varieties and to analyze correlations between trace elements and agronomic traits, we measured the selenium and carotenoid concentrations of 200 Chinese accessions by high-performance liquid chromatography and atomic lfuorescence spectrometry. Our analysis revealed that lutein concentration in 200 foxtail milet accessions folowed normal distribution and average was 3.1 μg g–1. The mean value of zeaxanthin concentration in 200 accessions was 8.6 μg g–1. Lutein and zeaxanthin concentrations were higher in the foxtail milet from Liaoning than in varieties from other locations, with averages of 10.0 and 3.5 μg g–1, respectively. The average measured selenium concentration was 100.3 μg kg–1. The highest average selenium concentration, 110.3 μg kg–1, was found in varieties from Shanxi. Varieties from Inner Mongolia had the lowest average selenium concentration, 84.7 μg kg–1, which was signiifcantly lower (P<0.05) than that of Shanxi. Selenium con-centrations of 23 varieties were higher than 117.9 μg kg–1, accounting for 11.5% of the total, thereby were considered to be enriched in selenium. In addition, we identiifed 29 lutein-enriched varieties (>4.27 μg g–1) and 30 zeaxanthin-enriched ones (>12.63 μg g–1), which corresponded to 14.5 and 15% of tested accessions, respectively. Correlation analysis revealed that selenium concentration was signiifcantly positively correlated with spikelet length (P<0.01), while zeaxanthin concentration was signiifcantly correlated with grass weight (P<0.05) and spikelet length (P<0.01). No correlation was found between lutein concentration and agronomic characters, selenium content or zeaxanthin content. Our results should contribute substantialy to the selection of suitable varieties for the development of plants with desired levels of these nutritionaly important elements. These results wil signiifcantly contribute towards selection of the most suitable varieties for obtaining plants with desired levels of these nutritionaly important elements.