Comparative proteomic analysis provides new insights into ear leaf senescence of summer maize (Zea mays L.) under ifeld condition

摘要

As the most important organ in plant photosynthesis, the leaf plays an important role in plant growth and development. Leaf senescence is associated with fundamental changes in the proteome. To research the molecular mechanisms of leaf senescence, protein expression in senescing maize ear leaves grown under ifeld conditions was analyzed using two-di-mensional gel electrophoresis and matrix-assisted laser desorption/ionisation time-of-lfight/time-of-lfight mass spectrometry (MALDI-TOF/TOF MS). A total of 60 senescence-associated proteins were identiifed. The identiifed proteins are involved in many biological processes, especialy energy, metabolism and protein synthesis. Several of the identiifed proteins have not been previously reported as senescence-associated, including glycine-rich RNA-binding protein.