Hydroxyapatite (HAP) ceramic with orderly micro-patterned surface were fabricated by transferring micro-groove patterns from an aluminum alloy template, which was carved by an ultrasound-driven microprobe. The surface morphologies, phase compositions, and water wettability of samples were respectively analyzed with scanning electron microscope (SEM), X-ray diffraction (XRD), and contact angle measurements. The groove widths of three-kind samples were about 20, 40 and 60 μm, in lined with the designed values. Water wettability of the grooved HAP surfaces was found to be significantly increased by decreasing groove-width. Furthermore, the influences of the grooved HAP surfaces on the behavior of osteoblasts (MC3T3-E1) were examined by measuring cell orientation and counting the cell number. The results showed that the cells were oriented along the groove direction, and the cell orientation angles decreased with a decrease in groove-width, whereas no significant difference in the number of osteoblasts was observed. In summary, the groove-width of micro-patterned HAP has a distinct effect on the directed growth of osteoblast cells.%利用超声探针在铝合金圆片上雕刻微米沟槽图案作为模板,并成功地将此沟槽图案转移到羟基磷灰石(HAP)陶瓷表面上.扫描电子显微镜(SEM)表征结果显示3种沟槽宽度分别为20、40和60μm,与预先设计沟槽的尺寸一致；接触角测量结果表明,沟槽化HAP表面的纯水润湿性随沟槽宽度的减小而明显提高.对体外培养在三种沟槽尺寸HAP表面成骨细胞(MC3T3-E1)的形态观察、细胞核染色计数以及细胞取向角测量统计分析的结果表明,沟槽化的HAP表面具有引导成骨细胞沿沟槽方向铺展的作用,而且这种引导作用随着沟槽变窄而显著增强,但这种引导作用并没有对成骨细胞的早期(30 h)增殖行为产生影响.
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