Fusarium solani Activates Dectin-1 in Experimentally Induced Keratomycosis

摘要

In this study,the response of Dectin-1 on macrophages to Fusarium solani(F.solani) and the expression patterns of Dectin-1 in experimentally F.solani-induced keratomycosis were investigated.Peritoneal macrophages isolated after intraperitoneal injection of sodium thioglycollate were co-cultured with laminarin and spores of F.solani for 12 h.The expression levels of Dectin-1 and CARD9 were detected by immunofluorescence and real-time quantitative polymerase chain reaction.A mouse model of fungal keratitis was established by substromal inoculation with spores of F.solani.Corneal lesions and inflammatory responses were observed by slit-lamp and histopathology at 1,2,3,5,and 7 day(s) after infection.Dectin-1 expression was significantly upregulated on macrophages stimulated by spores of F.solani.Dectin-1 was not detected in normal corneas of C57 BL/6 mice,but detected in infected corneas from the first day after inoculation,with high m RNA levels observed on days 2 and 3.CARD9,a key transducer of Dectin-1 signaling,was also upregulated in infected corneas.In conclusion,Dectin-1 is an important recognition receptor in F.solani-induced keratitis,but the molecular mechanisms warrant further investigation.Key