酚酸类物质对玉米幼苗生长及生理特性的影响

摘要

为了研究酚酸类物质对玉米幼苗生长和生理特性的影响,以阿魏酸、对香豆酸、对羟基苯甲酸、丁香酸、香草酸5种酚酸类物质为研究对象,分别采用0.01,0.10,1,10,50和100 mg·L-1的质量浓度对玉米进行处理,测定不同质量浓度的5种酚酸类物质处理后玉米幼苗株高、主根长、超氧化物歧化酶(SOD)活性、过氧化物酶(POD)活性、丙二醛(MDA)含量、可溶性蛋白质含量和可溶性糖含量等各项生长生理指标,试验结果表明,不同质量浓度的5种酚酸类物质处理后玉米幼苗苗高、主根长、可溶性蛋白质含量、POD活性和SOD活性均降低,0.01,0.1,1,10 mg·L-1的对羟基苯甲酸处理玉米幼苗可溶性蛋白质含量与对照间均达到了显著性水平(P＜0.05),0.1 mg·L-1的对香豆酸处理玉米幼苗POD活性显著低于对照(P＜0.05);阿魏酸、对香豆酸、对羟基苯甲酸、香草酸处理玉米幼苗CAT活性均降低;阿魏酸、对香豆酸、丁香酸处理玉米幼苗可溶性糖含量升高,对羟基苯甲酸处理降低.通过综合敏感指数可知,5种酚酸类物质对玉米幼苗抑制效应大小顺序为:对羟基苯甲酸＞香草酸＞阿魏酸＞对香豆酸＞丁香酸.%Experiments were conducted to study the effects of ferulic acid,p-coumaric acid,syringicacid,p-hydroxybenzoic acid and vanillic acid of different concentrations on the growth and physiology of maize seedlings.Maize seedlings were planted in box and were treated with 45 mL of 0.01,0.1,1,10,50,100 mg · L-1 of the five kinds of phenolic acids at 10 day intervals.The shoot height,root length,activities of superoxidase (SOD),catalase (CAT) and peroxidase (POD),Malondialdehyde (MDA),content of soluble protein and sugar were determined.The results showed that five kinds of phenolic acids all inhibited the growth of maize seedlings,reduced the activities of SOD,POD,soluble protein content,shoot height,and root length.The content of soluble protein of seedlings had more significant decrease under 0.01,0.1,1,10 mg · L-1 of p-hydroxybenzoic acid,p-coumaric acid at 0.1 mg · L-1 had more significant inhibitions than other treatments.In addition,compared with the control without ferulic acid,p-coumaric acid,p-hydroxybenzoic acid,vanillic acid,the activitie of CAT decreased,while it increased under the treatment of syringic acid.The content of soluble suger increased under the treatment of ferulic acid,p-coumaric acid,increased syringic acid,and vanillic acid,while it decreased under the treatment of p-hydroxybenzoic,the order is p-hydroxybenzoic acid ＞vanillic acid ＞ ferulic acid ＞ p-coumaric acid ＞ syringic acid.