本研究以小麦DH群体(花培3号×豫麦57)为材料,在水分胁迫及非胁迫条件下测定SOD、POD、CAT活性及MDA含量并进行QTL分析.结果表明:20％ PEG-6000浓度下亲本间抗旱系数差异最大,为最适胁迫浓度;在两种水分条件下,检测到10个QTL,分布在1A、1B、2D、3B、6A、6B和6D染色体,单个QTL贡献率为7.41％～15.57％;其中正常水分条件下检测到6个QTL,渗透胁迫下检测到4个QTL;其中5个主效QTL:QSOD-6D.OS、QPOD-2D.NW、QPOD-1B.OS、QPOD-2D.OS和QCAT-6B.NW,贡献率超过10％,可为小麦抗旱分子标记辅助育种提供理论依据.%The changes of antioxidant enzyme activity and malondialdehyde(MDA)content can reflect the drought resistance of wheat in the water stress environment.A doubled haploid (DH)population with 168 lines,which was derived from a cross between two common wheat varieties‘ H uapei 3’ and‘ Yumai 57 ’,was used to measure the SOD,POD,CAT activities and MDA content under normal water(NW)and osmotic stress(OS)treatments in the study.We determined that the osmotic stress with concentration of 20％ of PEG-6000 was the suitable treatment concentration,at which the parents had the most significant differences of drought resistance coefficient.A total of 10 QTLs were detected under two water regimes,among which 6 QTLs were for NW and 4 QTLs were for OS.They were located on chromosomes 1A,1B,2D,3B,6A,6B and 6D,respectively,with 7.41％ ～ 15.57％ of single QTL contribution rate.Five main-effect QTLs were identified,which were:QSOD-6D.OS,QPOD-2D.NW,QPOD-1B.OS,QPOD-2D.OS and QCAT-6B.NW.The study provided theoretical basis for the molecular marker assisted breeding of drought resistance in wheat.
展开▼
