HPLC法测定苦蘵中酸浆苦味素D的含量

摘要

A method was established for the determination of physalin D in the calyx of Physalis angulata L.A Waters Symmetry C18column(4.6×250 mm,5 μm)and mobile phase of acetonitrile-water(25:75)as well as 227 nm UV absorption at 25 ℃,by high performance liquid chromatography.The calibration curve of physalin D was in good linear relationship over the range of 10~100 μg·mL-1(r=0.999 8).The average recovery was 98.7%,and RSD(n=3)was 1. 8%.Therefore,this method is simple,quick,sensitive,accurate,and reproducible and can could be used to control the quality of Physalis angulata L.%建立了一种测定苦蘵中酸浆苦味素D含量的高效液相色谱法.采用 Waters Symmetry C18(4.6 × 250 mm,5 μm)色谱柱,以流动相为乙腈-水(25:75);检测波长为227 nm;柱温为25 ℃.结果表明:酸浆苦味素D浓度在10~100 μg·mL-1浓度范围呈良好的线性关系(r=0.9998),平均加样回收率(n=3)为98.7 %,RSD为1. 8%.说明本方法简便、准确、重复性好,可用于苦蘵药材的质量控制.