Mechanism of Weijing Decoction in the treatment of non-small cell lung cancer based on network pharmacology

摘要

Objective:To explore the potential mechanism of Weijing decoction in the treatment of non-small cell lung cancer based on the network pharmacology.Methods:The active constituent was collected based on the TCMSP database and the articles from CNKI and Pubmed databases.The targets of the active constituent were predicted by TCMSP database and Swiss Target Prediction.The drug-active components-targets network was built by utilizing Cytoscape3.7.2.OMIM,DrugBank,GeneCards and TTD databases were searched for the genes of NSCLC.Drug targets and disease targets were mapped and the protein-protein interaction(PPI)was acquired from the online STRING database.The hub genes were screened out through MCODE plug-in and the intersection targets were analyzed by Clue GO plug-in for GO functional enrichment and KEGG signaling pathway enrichment.Results:According to the threshold of OB≥30%and DL≥0.18,a total of 33 active components were selected.With 85 unique targets predicted,a total of 619 genes of NSCLC were obtained and 54 intersection targets were displayed through Venn diagram,among which the hub genes were EGFR、Casp3、MAPK8、PTGS2、JUN、TNF、JAK2,etc.GO enrichment analysis demonstrated vividly that the biological processes of Weijing decoction for NSCLC mainly focus on extrinsic apoptotic signaling pathway,nuclear receptor activity、positive regulation of blood vessel diameter.KEGG analysis illustrated that Weijing decoction might have an influence on Apoptosis signaling pathway,p53 signaling pathway,VEGF signaling pathway and so on.Conclusion:This article analyzed the main active constituent,hub genes,biological process and signaling pathways which were related to NSCLC on the basis of network of pharmacology,indicating that NSCLC can be treated by Weijing decoction through“multi-target and multi-pathway”.