Silencing lncRNA CTD-2510F5.4 inhibits the proliferation, invasion and RACGAP1 expression of Huh-7 human hepatocellular carcinoma cells

摘要

Objective:To study the effect of lncRNA CTD-2510F5.4 silencing on the proliferation and invasion of huh-7 human hepatocellular carcinoma cells and its possible regulatory relationship with RACGAP1.Methods:The TCGA(The Cancer Genome Atlas)database was used to analyze the expression of lncRNA CTD-2510F5.4 in liver cancer and normal tissues and the correlation with the expression of RACGAP1.CTD-2510F5.4 silencing test was performed on Huh-7 human liver cancer cells,and PCR was used to verify its silencing efficiency.CCK8,Transwell,flow cytometry were used to detect cell proliferation,invasion,and apoptosis,RTPCR,and Western blot.Detect the expression level of RACGAP1.Results:In the TCGA database,lncRNA CTD-2510F5.4 was highly expressed in liver cancer tissues,and it was positively correlated with the expression of RACGAP1(R=0.85).After silencing CTD-2510F5.4,the proliferation and invasion of huh-7 human liver cancer cells were inhibited,apoptosis increased,and the mRNA and protein expression of RACGAP1 were downregulated.Conclusion:lncRNA CTD-2510F5.4 may be the oncogenic lncRNA of liver cancer,and RACGAP1 may be the target of lncRNA CTD-2510F5.4.Silencing CTD-2510F5.4 can inhibit the proliferation and invasion of huh-7 human liver cancer cells,and promote apoptosis,which may be achieved by inhibiting the expression of RACGAP1.