Study on the protective effect of icariin in different concentrations on the inflammatory response of osteoblasts in fetal rats

摘要

Objective:To verify whether icariin has drug toxicity to osteoblasts and its pre protective effect on inflammatory osteoblasts induced by lipopolysaccharide.Methods:(1)The osteoblasts of newborn SD rats were extracted,cultured and passaged.By observing the morphology of osteoblasts and ALP staining,the activity and proliferation of osteoblasts were determined.(2)CCK-8 method was used to observe the effect of Icariin on osteoblast activity.(3)Different concentrations of lipopolysaccharide(LPS)were used to induce inflammatory osteoblasts in fetal rats.CCK-8 method was used to select the best concentration for induction.(4)This experiment was divided into control group,low concentration group,middle concentration group and high concentration group.CCK-8 method was used to observe whether icariin could protect osteoblasts from inflammatory reaction.Results:(1)The number of osteoblasts in the third generation increased,the shape of osteoblasts overlapped like tiles,most of osteoblasts grew in the center,the center was dense and the specific shape was difficult to see.After ALP staining,the positive cells showed gray black granules in cytoplasm and irregular cell body shape under inverted microscope;(2)DWhen the concentration of icariin was lower than 1μg/ml,it had no significant effect on osteoblasts(P<0.05);when it was higher than 10μg/ml,icariin had no significant effect on osteoblasts(P<0.05);(3)When the concentration of lipopolysaccharide was higher than 80μg/ml,there was a significant trend of inflammatory damage to osteoblasts,which was statistically significant(P<0.01).Therefore,80μg/ml was selected as the best injury concentration in this experiment;(4)When the concentration of icariin was lower than 1μg/ml,there was no significant pre protective effect on the inflammatory response of osteoblasts(P<0.05);when it was higher than 10μg/ml,there was significant pre protective effect on the inflammatory response of osteoblasts(P<0.05).Conclusion:When the concentration of icariin reaches a certain level,it can promote the proliferation of osteoblasts and has a certain pre protective effect on inflammatory osteoblasts.