Study on the Potential Mechanism of Drug Pair 'Honeysuckle-Astragalus' on COVID-19 based on Network Pharmacology

摘要

Objective:To explore the potential mechanism of drug pair "Honeysuckle-Astragalus" on 2019-nCoV using network pharmacology.Methods:The active compounds of Honeysuckle-Astragalus were searched by TCMSP database platform and BATMAN-TCM platform, and the targets related to the active compounds were searched and screened out through the database. The targets related to 2019-nCoV were screened out through OMIM and GeneCards databases. The network diagram of "drug-component-disease-target" was constructed by using the Cytoscape software, and the PPI network diagram of target protein interaction was constructed by using the STRING database. DAVID database was used for gene ontology(GO) analysis and Kyoto encyclopedia of genes and genomes(KEGG)pathway enrichment analysis.Results:23 active components of Honeysuckle, 20 active components of Astragalus and 10 targets of intersection of active components and 2019-nCoV were screened, mainly including NOS3, DPP4, TN, FIL6, etc. Using DAVID database analysis and taking P<0.05 as the screening criteria, 85 biological processes were obtained, of which the first 20 were related to processes such as response to antibiotic, lipopolysaccharide-mediated signaling pathway, negative regulation of cell proliferation, etc. Twenty-six signal pathways were screened, among which the first 20 were related to Malaria, Herpes simplex infection, TNF signaling pathway, etc. Conclusion: Honeysuckle-Astragalus may have the characteristic of multiple components, multiple targets and multiple pathways in the treatment of 2019-nCoV,and its mechanism of action may be to treat 2019-nCoV through antiviral infection and repair of lung injury, which provides a theoretical basis for the treatment of 2019-nCoV with honeysuckle-Astragalus in clinic.