Explore the mechanism of Danshen on coronary heart disease based on network pharmacology

摘要

Objective: To analyze the active mechanism of salvia miltiorrhiza on coronary heart disease and its target mechanism. Methods: An important systematic pharmacological technology platform (TCMSP) database was used to screen potential chemically active substances and related target proteins in Salvia miltiorrhiza. The genome annotation database platform (Genecards) was used to predict the targets of coronary heart disease. The uniprot database was used to query and Corresponding gene names, with the help of Cytoscape (3.7.2) software, to build a visualization of the "drug-disease-target" network diagram, construct a protein interaction network through the String database platform, and then use the Bioconductor platform and R language GO enrichment Analysis and KEGG enrichment analysis. Results: Through screening, 65 species of salvia miltiorrhiza, 54 key chemicals related to coronary heart disease, and 50 common targets of salvia miltiorrhiza and coronary heart disease were obtained. The core genes of PPI were ESR1, CCND1, FOS, NCOA1, and APP. , NR3C1, AR, NCOA2, RELA, EGFR, IL6, NYC, PGR, PPARG, RB1, CASP3;obtained 73 GO biological functions including steroid hormone receptor activity, nuclear receptor activity, transcription factor activity, etc., and 19 Related signaling pathways involve endocrine resistance;PI3K-Akt signaling pathway;apoptosis and so on. Conclusion: Salvia miltiorrhiza treatment of coronary heart disease reflects the characteristics of multi-component and multi-target of traditional Chinese medicine. It can regulate the occurrence and development of coronary heart disease through various channels, and provides ideas and basis for subsequent experiments.