Evaluation of vitaminD3 levels in primarySjogren's syndrome patients and its correlation with severity and immune function

摘要

Objective:To study the vitamin D3 levels in primary Sjogren's syndrome patients and its correlation with severity and immune function.Methods:Active pSS patients, stable pSS patients and healthy volunteers were chosen for study. Peripheral blood was collected, mononuclear cells were isolated and percentages of CD27high plasma cells and CD27+ memory B cells were detected; serum was collected and contents of 1,25(OH)2D3, TNF-α, IL-6, IL-10 and IL-17 were detected.Results:(1) 1,25(OH)2D3 contents in serum as well as CD27high plasma cell and CD27+ memory B cell contents in peripheral blood of pSS group were lower than those of control group; serum TNF-α, IL-6 and IL-17 contents were higher than those of control group and IL-10 contents were lower than those of control group; (2) 1,25(OH)2D3 contents in serum as well as CD27high plasma cell and CD27+ memory B cell contents in peripheral blood of active pSS patients were lower than those of stable pSS patients; serum TNF-α, IL-6 and IL-17 contents were higher than those of stable pSS patients and IL-10 contents were lower than those of stable pSS patients; (3) 1,25(OH)2D3 level was positively correlated with contents of CD27highplasma cells, CD27+ memory B cells and IL-10, and negatively correlated with TNF-α, IL-6 and IL-17 contents.Conclusion:Abnormal reduction of Vitamin D3 levels is involved in the pathogenesis of primary Sjogren's syndrome and closely related to the severity and immune function of the disease.