Relationship between high risk HPV infection and potential malignant biological behavior of patients with cervical intraepithelial neoplasia

摘要

Objective: To study the relationship between high risk HPV infection and potential malignant biological behavior of patients with cervical intraepithelial neoplasia. Methods: Patients with cervical intraepithelial neoplasia treated in our hospital from May 2012 to May 2014 were chosen for study; 40 cases of patients each in CIN I stage, CIN II stage and CIN III stage were screened; HPV subtypes as well as mRNA contents of proliferation related genes and chromosome related genes were detected. Results: (1) HPV subtypes: there were no differences in positive rates and contents of HPV-31, 33, 51, 52 and 58 of patients in different CIN stages; there were differences in positive rates and contents of HPV-16 and 18, and the higher the CIN stage is, the higher the positive rates and contents of HPV-16 and 18 are; (2) Proliferation related genes: mRNA contents of proliferation genes IMP3, MUC1 and TS in cervical tissue of patients with high risk HPV infection displayed higher expressions; mRNA contents of tumor suppressor genes p16ink4a and p53 displayed lower expressions; (3) Chromosome related genes: mRNA contents of MCM2, MCM7, hTERC, hTERT, hTP1 and CA-IX in cervical tissue of patients with high risk HPV infection displayed higher expressions. Conclusion: HPV-16 and 18 are high risk HPV of patients with cervical intraepithelial neoplasia and will cause increased expressions of proliferation genes and inhibited expressions of tumor suppressor genes, and affect chromosome replication and extension process.