Effect of artemisinin combined with cisplatin intervention on epithelial-mesenchymal transition, angiogenesis and ATP generation in MGC-803 gastric cancer cell lines

摘要

Objective:To study the effect of artemisinin combined with cisplatin intervention on epithelial-mesenchymal transition, angiogenesis and ATP generation in MGC-803 gastric cancer cell lines.Methods: MGC-803 gastric cancer cell lines were cultured and divided into control group, cisplatin group, artemisinin group, cisplatin + artemisinin group, cisplatin + artemisinin + NAC group and NAC group, and after different conditions of treatment, cell viability, apoptosis rate as well as levels of epithelial-mesenchymal transition molecules, angiogenesis molecules and ATP were determined.Results: Cell viability, ATP generation as well as VEGFA, VEGFB, VEGFC, N-cadherin and Vimentin levels of cisplatin group, artemisinin group and cisplatin + artemisinin group were lower than those of control group, and early apoptosis rate, late apoptosis rate and E-cadherin levels were higher than those of control group; cell viability, ATP generation as well as VEGFA, VEGFB, VEGFC, N-cadherin and Vimentin levels of cisplatin + artemisinin group were lower than those of cisplatin group and artemisinin group, and early apoptosis rate, late apoptosis rate and E-cadherin level were higher than those of cisplatin group and artemisinin group. E-cadherin level of cisplatin + artemisinin + NAC group was lower than that of cisplatin + artemisinin group, and ATP generation as well as VEGFA, VEGFB, VEGFC, N-cadherin and Vimentin levels were higher than those of cisplatin + artemisinin group.Conclusion:Artemisinin combined with cisplatin intervention can synergistically exert the inhibitory effect on epithelial-mesenchymal transition, angiogenesis and ATP generation in MGC-803 gastric cancer cell lines, and the inhibiting effect is partially realized by increasing the generation of reactive oxygen species.