Changes of osteosarcoma cell biological behavior afterId1 gene silencing by siRNA

摘要

Objective:To study the changes of osteosarcoma cell biological behaviors proliferation, migration and invasion afterId1gene silencing by siRNA.Methods:Osteosarcoma HEK293 cell lines were cultured and transfected with Id1-siRNA and NC-siRNA respectively, cell proliferation, migration and invasion were detected after 24 h and 48 h of transfection, and the expression levels of proliferation-promoting genes, proliferation-inhibiting genes as well as migration and invasion-related genes in cells were detected after 48 h of transfection. Results:After 24 h and 48 h of transfection, cell OD value and the number of invasive cells of Id1-siRNA group were significantly lower than those of NC-siRNA group, and scratch area was significantly larger than that of NC-siRNA group; after 48 h of transfection, hnRNP A2, hnRNP B1, S100A6, RUNX2, Aurora-A, Grb2, Gab2 and Rap2a expression levels of Id1-siRNA group were significantly lower than those of NC-siRNA group, and p53, TAp73 and IGFBP5 were significantly higher than those of NC-siRNA group.Conclusions:Id1 gene silencing by siRNA can inhibit osteosarcoma cell proliferation, migration and invasion.