Exploration of the inhibiting effect of Notch-1 blocker on tumor growth in osteosarcoma mouse models as well as related molecular mechanisms

摘要

Objective:To explore the inhibiting effect of Notch-1 blocker on tumor growth in osteosarcoma mouse models as well as related molecular mechanisms.Methods: SCID mice were selected as experimental subjects, and osteosarcoma mouse models were built through subcutaneous injection of osteosarcoma cells and divided into GSI group and PBS group who received GSI and PBS intervention respectively. 0 d, 5 d, 10 d, 15 d and 20 d after intervention, tumor tissue volume was detected; 20 d after intervention, the contents of pro-apoptosis, pro-proliferation and invasion-related molecules in tumor tissue were detected.Results: On the 0 d of intervention, tumor volume of GSI group was not significantly different from that of PBS group; 5 d, 10 d, 15 d and 20 d after intervention, tumor volume of GSI group was significantly lower than that of PBS group; 20 d after intervention, the contents of pro-proliferation molecules AEG-1, Orai1, STIM1, PAK5, RIPK4 andβ-catenin as well as invasion-promoting molecules Gab2, DLK1, Scr and B7-H3 in tumor tissue of GSI group were significantly lower than those of PBS group, and the contents of pro-apoptosis molecules RanBP9, PD-1, PD-L1 and PD-L2 as well as invasion-inhibiting molecules TIMP1 and TIMP2 were significantly higher than those of PBS group.Conclusion:Notch-1 blocker can inhibit tumor growth in osteosarcoma mouse models, and the molecular mechanisms involved in the process include reducing the generation of pro-proliferation molecules and invasion-promoting molecules and increasing the generation of pro-apoptosis and invasion-inhibiting molecules.