Analysis of the expression of miRNAs and downstream target genes in gastric cancer tissue and exploration of its relationship with clinicopathologic stage

摘要

Objective:To study and analyze the expression of miRNAs and downstream target genes in gastric cancer tissue and its relationship with clinicopathologic stage.Methods:Patients diagnosed with gastric cancer in our hospital from April 2012 to Decempber 2014 were selected for study, and gastric cancer tissue and paracancer tissue were collected to detect the expression of miRNAs as well as the contents of proteins encoded by drug resistance-related genes, proliferation-related genes and EMT-related genes.Results: miR-21, miR-106a, miR-192, miR-194, miR-210 and miR-215 expression in gastric cancer tissue was significantly up-regulated, miR-30a, miR-125, miR-149, miR-194, miR-205 and miR-365 expression was significantly down-regulated, and the higher the TNM stage of tumor, the more significant the change of the expression of above miRNAs; the trend of miR106 and miR-30a were the most significant, the former was up-regulated by 4.38 times and the latter was down-regulated by 0.23 times; P-gP, GST-π, CACNA2D1, RPL23, Hsp27, ZNF139, Mcmp4, OPCML, N-cadherin and Vimentin contents in gastric cancer tissue were significantly higher than those in paracancer tissue, and E-cadherin content was significantly lower than that in paracancer tissue; miR106 expression level was positively correlated with P-gP, GST-π, CACNA2D1, RPL23, Hsp27, ZNF139, Mcmp4, OPCML, N-cadherin and Vimentin contents and negatively correlated with E-cadherin content; miR-30a expression level was negatively correlated with P-gP, GST-π, CACNA2D1, RPL23, Hsp27, ZNF139, Mcmp4, OPCML, N-cadherin and Vimentin contents and positively correlated with E-cadherin content.Conclusion: miR106 expression significantly increases and miR-30a expression significantly decreases in gastric cancer tissue, and miR106 and miR-30a can regulate the expression of drug resistance genes, proliferation genes and EMT genes.