Protective effect of recombinant human erythropoietin on liver and kidney injury as well as endoplasmic reticulum stress caused by infection

摘要

Objective: To study the protective effect of recombinant human erythropoietin on liver and kidney injury as well as endoplasmic reticulum stress caused by infection. Methods: Male Wistar rats were randomly divided into negative control group, LPS group and LPS+EPO group, LPS group and LPS+EPO group received injection of LPS via caudal vein to establish infection models, and LPS+EPO group received injection of rHuEPO via caudal vein for intervention; before intervention as well as 6 and 12 h after intervention, serum was collected to detect TNF-α, iNOS, BUN, Cr, ALT and AST contents; 12 h after intervention, liver and kidney tissue was collected to detect mRNA contents of endoplasmic reticulum stress molecules (GRP78, CHOP and Caspase-12). Results: After model establishment and before rHuEPO intervention, serum TNF-α, iNOS, BUN, Cr, ALT and AST contents of LPS group and LPS+EPO group had no differences and were higher than those of negative control group; 6 and 12 h after intervention, serum TNF-α, iNOS, BUN, Cr, ALT and AST contents of LPS+EPO group were lower than those of LPS group; 12 h after intervention, mRNA contents of GRP78, CHOP and Caspase-12 in liver and kidney tissue of LPS+EPO group were lower than those of LPS group. Conclusions: Recombinant human erythropoietin has protective effect on liver and kidney injury as well as endoplasmic reticulum stress caused by infection.