Gadd45α expression in preeclampsia placenta and the effect of Gadd45α on trophoblast HTR8/Svneo

摘要

Objective:To study the expression of Gadd45α in preeclampsia placenta and the regulating effect of Gadd45 knockdown on trophoblast HTR8/Svneo.Methods:Preeclampsia placenta tissue and normal placenta tissue were collected, and mRNA contents and protein contents of Gadd45α were detected by fluorescent quantitative PCR and Western blotting respectively; trophoblast cells HTR8/Svneo were cultured and after transfection of Gadd45αsiRNA, cell invasion ability and expression of invasion-assiotiated molecules were detected. Results:mRNA content and protein content of Gadd45α in preeclampsia placenta tissue were higher than those in normal placenta tissue; after transfection of Gadd45α siRNA, mRNA content and protein content of Gadd45α in HTR8/Svneo cells significantly decreased, and the number of invasive cells as well as expression of MMP1, MMP2, MMP3 and MMP9 significantly increased. Conclusion: The expression of Gadd45α in preeclampsia placenta abnormally increases; inhibting the expression of Gadd45α in trophoblasts HTR8/Svneo can promote invasion and increase the expression of MMPs molecules.