Influence of paeonol combined with γ ray on glioma cell apoptosis and apoptosis gene expressiona

摘要

Objective: To study the influence of paeonol combined with γ ray on glioma cell apoptosis and apoptosis gene expression. Methods: Glioma C6 cells were cultured and divided into control group (treated with serum-free DMEM), ray group (exposed to 4 Gy γ ray), Pae group (treated with serum-free DMEM contains 4 μg/mL paeonol) and γ+Pae group (treated with serum-free DMEM contains 4 μg/mL paeonol and exposed to 4 Gy γ ray). The cell proliferation activity value as well as the expression of pro-apoptosis genes and anti-apoptosis genes was measured after the intervention. Results: After 6 h, 12 h, 18 h and 24 h of intervention, cell proliferation activity value of ray group, Pae group and γ+Pae group were greatly lower than that of control group, and cell proliferation activity value of γ+Pae group was obviously lower than that of ray group and Pae group. After 24 h of intervention, p21cip1, TRAIL, LRIG1, NPRL2 and SEPT7 mRNA expression in ray group, Pae group and γ+Pae group were notably higher than those in control group whereas PIAS3, CEACAM1, EZH2, MDM2, c-myc and Survivin mRNA expression were greatly lower than those in control group; p21cip1, TRAIL, LRIG1, NPRL2 and SEPT7 mRNA expression in γ+Pae group were remarkably higher than those in ray group and Pae group whereas PIAS3, CEACAM1, EZH2, MDM2, c-myc and Survivin mRNA expression were greatly lower than those in ray group and Pae group. Conclusion: Paeonol combined with γ ray can be more effective than γ ray alone in inducing the apoptosis of glioma cells, increasing the expression of pro-apoptosis genes and decreasing the expression of anti-apoptosis genes.