The relationship between osteopontin expression and cell invasion as well as angiogenesis in preeclampsia placenta tissue

摘要

Objective:To study the relationship between osteopontin expression and cell invasion as well as angiogenesis in preeclampsia placenta tissue.Methods:A total of 56 patients diagnosed with preeclampsia in our hospital between June 2014 and March 2016 were selected as preeclampsia group (PE group) and 80 normal subjects receiving antenatal examination and giving birth during the same period were selected as control group. After labor, the placenta tissue was collected, immunohistochemical staining kits were used to detect OPN expression and enzyme-linked immunosorbent assay kits were used to determine cell invasion and angiogenesis molecule expression.Results:Positive OPN rate in placenta tissue of PE group was significantly lower than that of control group; cell invasion molecules LAMA4, uPA, Rho and ROCK as well as angiogenesis molecules VEGF, PIGF and AGF protein expression in placenta tissue of PE group were significantly lower than those of control group, and cell invasion molecules LAMA4, uPA, Rho and ROCK as well as angiogenesis molecules VEGF, PIGF and AGF protein expression in OPN-positive placenta tissue were significantly higher than those in OPN-negative placenta tissue.Conclusion:Lowly expressed OPN in preeclampsia placenta tissue will inhibit the expression of cell invasion molecules and angiogenesis molecules so as to inhibit sertoli cell invasion and the angiogenesis in placenta tissue.