Effect of local injection of pigment epithelium-derived factor on angiogenesis, inflammatory response and apoptosis in the retina of diabetic rats

摘要

Objective: To study the effect of local injection of pigment epithelium-derived factor (PEDF) on angiogenesis, inflammatory response and apoptosis in the retina of diabetic rats. Methods:Adult male SD rats were selected as experimental animals and randomly divided into control group, DM group and PEDF group, DM group and PEDF group were made into diabetes models through intraperitoneal injection of streptozotocin, and PEDF group were given local injection of PEDF. The contents of angiogenesis molecules and inflammatory response molecules as well as the expression of apoptosis genes in retinal tissue were measured after intervention. Results: mTOR, VEGF, VEGFR, Ang-2, Tie-2, NF-κB, COX2, iNOS, SDF-1 and CXCR4 contents as well as ASK1, JNK, p38MAPK and Caspase-3 mRNA expression in retinal tissue of DM group were significantly higher than those of control group whereas Survivin and Bcl-2 mRNA expression were significantly lower than those of control group;mTOR, VEGF, VEGFR, Ang-2, Tie-2, NF-κB, COX2, iNOS, SDF-1 and CXCR4 contents as well as ASK1, JNK, p38MAPK and Caspase-3 mRNA expression in retinal tissue of PEDF group were significantly lower than those of DM group whereas Survivin and Bcl-2 mRNA expression were significantly higher than those of control group. Conclusion: Local injection of PEDF has inhibitory effect on the angiogenesis, inflammatory response and apoptosis in the retina of diabetic rats.