Correlation of SOD-Mn gene polymorphism with renal function and oxidative injury in patients with diabetic nephropathy

摘要

Objective:To study the correlation of SOD-Mn gene polymorphism with renal function and oxidative injury in patients with diabetic nephropathy.Methods: Patients who were diagnosed with diabetic nephropathy in Yingshan County People's Hospital between March 2014 and March 2017 were chosen as DN group, and healthy volunteers who received physical examination during the same period were selected as the control group. The SOD-Mn gene rs4880 locus polymorphism and oxidative stress molecule expression in peripheral blood as well as the contents of renal function indexes and oxidative stress indexes in serum were determined.Results: The constituent ratio of SOD-Mn gene CC genotype in DN group was lower than that in control group whereas the constituent ratio of CT+TT genotype was higher than that in control group; serum T-AOC contents as well as peripheral blood Nrf2 and SIRT1 expression intensity of DN group of patients with CC genotype and CT+TT genotype were significantly lower than those of control group whereas serum Scr, BUN, CysC,β2-MG, MDA, AOPP, 8-OHdG and 8-Iso-PGF2α contents as well as peripheral blood NOX4, NOX5, p38MAPK and NF-κB expression intensity were significantly higher than those of control group, and serum T-AOC content as well as peripheral blood Nrf2 and SIRT1 expression intensity of patients with CT+TT genotype was significantly lower than those of patients with CC genotype whereas serum Scr, BUN, CysC,β2-MG, MDA, AOPP, 8-OHdG and 8-Iso-PGF2α contents as well as peripheral blood NOX4, NOX5, p38MAPK and NF-κB expression intensity were significantly higher than those of patients with CC genotype. Conclusion: The mutation from SOD-Mn gene rs4880 locus C to T can aggravate the renal function injury and oxidative stress response in patients with diabetic nephropathy.