Correlation of c-Myc and PIK3R3 expression with invasion and migration genes in melanoma tissue

摘要

Objective:To study the correlation of c-Myc and PIK3R3 expression with invasion and migration genes in melanoma tissue.Methods: Surgical removed melanoma tissue and normal skin tissue left in full-thickness skin grafting in 95 Clinical Department, Fuzhou General Hospital between May 2015 and March 2017 were selected, the RNA in the tissue was separated and extracted, and then fluorescence quantitative PCR kit was used to determine the mRNA expression of c-Myc and PIK3R3 as well as invasion and migration genes.Results:c-Myc, PIK3R3, CXCL12, CXCR4, CXCR7, NRP1, MMP2, MMP9 and CatK mRNA expression in melanoma tissue were significantly higher than those in normal skin tissue while E-cadherin, TIMP1 and KISS1 mRNA expression were significantly lower than those in normal skin tissue; c-Myc mRNA expression in melanoma tissue was positively correlated with PIK3R3 mRNA expression; CXCL12, CXCR4, CXCR7, NRP1, MMP2, MMP9 and CatK mRNA expression in melanoma tissue with higher c-Myc expression were significantly higher than those in melanoma tissue with lower c-Myc expression while E-cadherin, TIMP1 and KISS1 mRNA expression were significantly lower than those in melanoma tissue with lower c-Myc expression.Conclusion:The high expression of c-Myc and PIK3R3 in melanoma tissue can promote the invasion and migration of cancer cells.