Correlation of TTF-1 and Napsin A expression in CT-guided percutaneous puncture tissue with the malignant biology of cancer cells

摘要

Objective: To study the correlation of thyroid transcription factor-1 (TTF-1) and Napsin A expression in CT-guided percutaneous puncture tissue with the malignant biology of cancer cells. Methods: The lung tissues that were obtained from CT-guided percutaneous puncture in Tongchuan People's Hospital between March 2015 and December 2017 were selected and pathologically diagnosed with lung cancer tissues (n=78) and benign tissues (n=40), the RNA was extracted, and then the expression levels of TTF-1, NapsinA, oncogenes and epithelial-mesenchymal transition (EMT) genes were determined. Results: TTF-1, Pim1, C-myc, Nr4a1, KLF4 and N-cadherin mRNA expression levels in lung cancer tissues were significantly higher than those in benign tissues whereas NapsinA, Rb, LKB1, HIPK2 and E-cadherin mRNA expression levels were significantly lower than those in benign tissues; Pim1 and C-myc mRNA expression levels in lung cancer tissues with high TTF-1 expression were significantly higher than those in lung cancer tissues with low TTF-1 expression whereas Rb and LKB1 mRNA expression levels were significantly lower than those in lung cancer tissues with low TTF-1 expression; HIPK2 and E-cadherin mRNA expression levels in lung cancer tissues with high NapsinA expression were significantly higher than those in lung cancer tissues with low NapsinA expression whereas Nr4a1, KLF4 and N-cadherin mRNA expression levels were significantly lower than those in lung cancer tissues with low NapsinA expression. Conclusion: The high expression of TTF-1 and the low expression NapsinA in CT-guided percutaneous puncture tissue can promote the cancer cell proliferation and EMT respectively.