NOV gene expression in osteosarcoma tissue and its correlation with cell proliferation and migration

摘要

Objective: To investigate the NOV gene expression in osteosarcoma tissue and its correlation with cell proliferation and migration. Methods: Osteosarcoma tissue and adjacent normal tissue from 97 patients with osteosarcoma who underwent surgical treatment in the Central Hospital of Xiaogan between May 2014 and August 2017 were selected as the osteosarcoma group and the normal control group respectively. Fluorescence quantitative PCR was used to detect the expression of NOV gene, proliferation-related genes and invasion-related genes in different tissue samples, and Pearson test was used to evaluate the NOV gene expression in osteosarcoma tissue and its correlation with cell proliferation and migration. Results:NOV mRNA expression in osteosarcoma group was significantly lower than that in normal control group; KISS-1 and TAp73 mRNA expresion in osteosarcoma group were lower than those in normal control group whereas VCP, FoxM1, RIPK4 and STIM1 mRNA expression were higher than those in normal control group; DLX1 and Sema6A mRNA expression inosteosarcoma group were lower than those in normal control group whereas EFEMP1, GPR56, SOX5 and CRYAB mRNA expression were higher than those in normal control group. Pearson test showed that the NOV gene expression in osteosarcoma was directly correlated with the expression of cell proliferation genes and invasion genes. Conclusions: NOV gene expression in osteosarcoma tissue is lower than that in adjacent normal tissue, and the specific expression is directly correlated to tumor cell proliferation and invasion activity, and can be as a reliable index to judge the malignant degree of osteosarcoma.