补中益气汤及益气升阳配伍对脾气虚大鼠药理效应的影响

摘要

Objective To study the pharmacological action of Buzhong Yiqi Decoction ( BYD) and qi-strengthening and yang-elevating herbal compatibility of Radix Astragali, Rhizoma Cimicifugae, and Radix Bupleuri on spleen-qi-deficiency rats. Methods Spleen-qi-deficiency rat models were established by the combined method of gastric gavage of the decoction of Radix et Rhizoma Rhei, swimming-induced strength exhaustion by swimming and starvation. Rats were randomized into normal control group, model group, BHD group (in the dosage of 12 g o kg-1 o d-1) , qi-strenglhening and yang-elevating herbs group (in the dosage of 4. 5 g o kg-1 o d-1) , and BHD group with qi-strengthening and yang-elevating herbs removed (in the dosage of 7. 5 g o kg-1 o d-1). Gastric mucosal blood flow, serum creatine kinase activity, serum D-xylose content and amylase activity in rats were examined 14 days after the medication. Results Compared with the model group, BHD had an effect on increasing gastric mucosal blood flow, serum D-xylose content and amylase activity, and creatine kinase activity (P <0. 05). Qi-strengthening and yang-elevating herbs, and BHD with Qi-strengthening and yang-elevating herbs removed had an effect on increasing amylase activity ( P < 0. 05 ) , but had no effect on other examination indexes ( P > 0. 05 ). Conclusion BHD can effectively improve the pharmacological parameters of spleen-qi-deficiency rats, and qi-strengthening and yang-elevating herbal compatibility is the necessary composition of BHD.%[目的]观察补中益气汤及益气升阳配伍(黄芪—升麻、柴胡)对脾气虚大鼠药理效应的影响.[方法]采用大黄法、游泳力竭法、饥饿法3因素复合的方法复制脾气虚大鼠模型,造模成功后,将动物随机分成正常对照组、脾气虚模型组、补中益气汤组(12 g·kg-1·d-1)、益气升阳组(4.5g·kg-1·d-1)和原方去益气升阳组(7.5 g·kg-1·d-1),给药14d后测定各组大鼠胃黏膜血流量、肌酸激酶活性、D-木糖血清浓度及淀粉醇活性.[结果]与脾气虚模型组比较,补中益气汤组能显著提高脾气虚大鼠胃黏膜血流量、血清D-木糖浓度、淀粉酶活性及血清肌酸激酶活性(均P ＜0.05),而益气升阳组和原方去益气升阳组除了能显著提高淀粉酶活性外(P＜0.05),对其他3项指标均无显著影响(P＞0.05).[结论]补中益气汤能有效改善脾气虚大鼠的相关药理指标,益气升阳配伍是方中不可或缺的重要组成部分.