自拟中药复方对耐药性金黄色葡萄球菌的抑菌作用研究

摘要

Objective To study the antibacterial action of self-prescribed Chinese herbal compound formula (mainly composed of Radix Scutellariae, Fructus Forsythiae and Herba Menthae) against methicillin-resistant Staphylococcus aureus (MRSA) in vitro and in vivo. Methods In-vitro MRS A test and in-vivo MRSA experiment were carried out to observe the antibacterial action of self-prescribed Chinese herbal compound formula. Results The results of in-vitro MRSA test showed that the minimum inhibitory concentration of high- and low-dose self-prescribed Chinese herbal compound formula against MRSA was 3. 25 and 2. 23 mg /mL respectively. The results of in-vivo MRSA test showed that the death rate of mice infected with MRSA markedly decreased after treatment with 5. 20 g · kg-1 · d -1 of Radix Scutellariae and 5. 72 g · kg-1 · d-1 of self-prescribed Chinese herbal compound formula. Conclusion Self-prescribed Chinese herbal compound formula shows certain antibacterial action on MRSA.%[目的]研究自拟中药复方(由黄芩、连翘、薄荷组成)对耐药性金黄色葡萄球菌的体内、体外抑菌作用.[方法]通过体外对耐药性金黄色葡萄球菌(MRSA)的抑菌实验及体内对MRSA感染小鼠的保护作用,观察自拟中药复方的抑菌作用.[结果]体外实验结果表明:自拟中药复方高剂量对MRSA的最低抑菌浓度是3.25 mg/mL,低剂量的最低抑菌浓度是2.23 mg/mL.体内实验结果表明:黄芩高剂量组(5.20 g· kg-1·d-1)和自拟中药复方低剂量组(5.72 g·kg-1·d-1)均可显著降低耐药性金黄色葡萄球菌感染小鼠的死亡率(P＜0.05).[结论]自拟中药复方对耐药性金黄色葡萄球菌具有一定的抑菌作用.