电针对急性脑梗死大鼠肾功能的影响

摘要

Objective:To investigate the effects of electroacupuncture treatment on renal injury in rats with acute cerebral infarction (ACI).Methods:Seventy-two male Sprague-Dawley (SD) rats were randomly divided into three groups (n =24 in each):sham-operated group,model group and electroacupuncture group.The rat middle cerebral artery occlusion (MCAO) model for ischaemic stroke was established in model group and electroacupuncture group.The electroacupuncture group rats received electroacupuncture at acupoints of Neiguan (PC6) and Zusanli (ST36) daily 30 min after 24 h of model establishment for 2 weeks.On the first,third,seventh and fourteenth day after operation,6 rats from each group were randomly selected.The motor function was evaluated by footstep errors test.The creatinine (Cr),blood urea nitrogen (BUN) and cystatin C (Cys C) in the serum were detected.Morphological changes of kidneys were evaluated by Masson staining.Results:The behavioral score and the serum levels of Cr,BUN and Cys C in the electroacupuncture group were markedly lower than those in the model group on the 7th and 14th day post-operation (P ＜ 0.05),whereas no significant difference was noted in behavioral score and serum Cr and BUN contents on the first and third day between the two groups (P＞0.05).On days 7 and 14 after operation,there was a significant reduction in collagen deposition in renal tubular interstitium of ACI rats with electroacupuncture treatment.Spearman's correlation coefficient analysis demonstrated that the behavioral score was closely associated with the serum Cr,BUN and Cys C levels in ACI rats (P ＜0.05).Conclusion:Electroacupuncture at Zusanli and Neiguan could improve both motor function and renal function in rats with ACI.%目的:探讨电针治疗对急性脑梗死(ACI)大鼠肾功能的影响.方法:将72只SD雄性大鼠随机分为假手术组、模型组和电针组,每组24只.模型组和电针组采用Longa改良线栓法制备右侧大脑中动脉闭塞(MCAO)模型.术后24 h,电针组大鼠取双侧内关(PC6)、足三里(ST36)行电针治疗,每日1次,每次30 min,连续2周.分别于手术后第1、第3、第7和第14天,每组随机选取6只大鼠,采用脚步错误实验测定运动功能评分,检测血清肌酐(Cr)、尿素氮(BUN)、胱抑素C(Cys C)水平,Masson染色法观察大鼠肾组织病理学变化.结果:术后第7、第14天,电针组大鼠脚步错误评分及血清Cr、BUN、Cys C浓度均明显低于模型组(P＜0.05),而术后第1、第3天,两组脚步错误评分及血清Cr、BUN浓度比较,差异无统计学意义(P＞0.05).Masson染色显示,电针组和模型组大鼠术后第7、第14天肾间质可见明显胶原沉淀,且电针组明显少于模型组.Spearman相关分析显示,电针组、模型组大鼠的运动功能变化与肾功能改变有明显相关性(P＜0.05).结论:电针足三里和内关穴在改善ACI大鼠运动功能的同时还可改善肾功能.