多元醇法制备纳米银及其胆道感染常见细菌的抗菌性能

摘要

Objective:To study the effect of nano-silver prepared by polyol method on the treatment of biliary tract infection.Methods:The nano-silver particles were prepared by polyol method and characterized by transmission electron microscopy and particle size analyzer.The nano-silver particles with the smallest particle size were selected in vitro to detect the mininal inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of Escherichia coli,Pseudomonas aeruginosa and Enterococcus faecalis.Results:The particle size of nano-silver was decreased with the increases in the length of the polyol chain and the proportion of 3-aminopropanol.The nano-silver diameter could be controlled accurately between 8-20 nm.The nano-silver of 10.6 nm diameter was prepared by 220 ℃ polyethylene glycol as solvent.The MIC of the nano-silver particle to Escherichia coli,Pseudomonas aerμginosa,and Enterococcus faecalis was 9.76 mg/L,19.53 mg/L,and 39.06 mg/L,respectively.And the MBC of the nano-silver particle to these bacteria were 78.12 mg/L,3125.5 mg/L,and more than 5000 mg/L,respectively.Conclusion:Nano-silver particle was effective against the three kinds of bacteria,and had bactericidal action to Escherichia coli and Pseudomonas aerμginosa at a certain concentration.Therefore,it was capable to treat common biliary tract infection.%目的:研究纳米银的制备方法及其对胆道感染常见细菌的抗菌性能.方法:采用多元醇法制备纳米银颗粒,透射电镜和粒度分析仪对纳米粒子进行表征.选用粒径最小的纳米银颗粒在体外对大肠埃希菌、铜绿假单胞菌、粪肠球菌的最小抑菌浓度(MIC)值和最小杀菌浓度(MBC)进行检测,从而明确其抗胆道感染常见菌的性能.结果:多元醇链长增加和3-氨基丙醇比例增加,纳米银的粒径减小,纳米银粒径可以在8～20 nm精确控制.采用聚乙二醇为溶剂220℃制备得到10.6 nm的纳米银对大肠埃希菌、铜绿假单胞菌、粪肠球菌的MIC值分别为9.76 mg/L、19.53 mg/L、39.06 mg/L,而MBC值为78.12 mg/L、3 125.5 mg/L、大于5 000 mg/L.结论:从抑制细菌效果来说,纳米银对3种菌株均有效,从杀菌效果来说,纳米银对大肠埃希菌和铜绿假单胞菌在一定浓度下可以达到杀菌作用,纳米银对胆道感染常见细菌有效,可应用于胆道感染治疗.