铁皮石斛复方制剂的抗氧化和延缓衰老作用研究

摘要

目的 研究铁皮石斛复方制剂的抗氧化和延缓衰老作用.方法 通过二苯代苦味酰基自由基(DPPH)和羟自由基清除实验检测铁皮石斛复方制剂的体外抗氧化活性.利用秀丽线虫氧化应激模型,验证铁皮石斛复方制剂的体内抗氧化活性,同时在正常条件下检测铁皮石斛复方制剂对寿命的影响.在氧化应激条件下,测定铁皮石斛复方制剂对秀丽线虫活性氧(ROS)水平、超氧化物歧化酶(SOD)和过氧化氢酶(CAT)的活力以及丙二醛(MDA)含量的影响.结果 铁皮石斛复方制剂对DPPH和羟自由基均有较好的清除作用,在16.0 mg/mL质量浓度下对DPPH和羟自由基清除率均达到60%.铁皮石斛复方制剂能提高秀丽线虫在高浓度百草枯(50 mmol/L)氧化胁迫条件下的存活率,同时也能延长其在正常条件下的寿命.在低浓度百草枯(2 mmol/L)氧化胁迫下,铁皮石斛复方制剂能显著降低秀丽线虫ROS含量并增强SOD活力,但对CAT活力和MDA含量没有明显影响.结论 铁皮石斛复方制剂具有抗氧化活性,能提高秀丽线虫在氧化胁迫条件下的存活率并延长寿命,其机制可能与提高秀丽线虫SOD活力有关.%Objective To study the antioxidant and anti-aging effect of Dendrobium officinale formula in Caenorhabditis elegans models.Methods The antioxidant activity of D.officinale formula was first determined in vitro using DPPH and hydroxyl free radical scavenging assays.Then food clearance assay was performed to determine the appropriate dosages of D.officinale formula to be used in C.elegans.Survival rate and lifespan of C.elegans were then determined under oxidative stress induced by paraquat and under normal growth conditions,respectively.The antioxidant enzyme activities,reactive oxygen species (ROS) levels and malondialdehyde (MDA) content were measured in C.elegans exposed to paraquat.Results D.officinale formula was capable of scavenging both DPPH and hydroxyl free radicals in vitro.The formula increased the survival rate of C.elegans under oxidative stress induced by 50 mmol/L paraquat and extend the lifespan under normal growth conditions.When C.elegans was exposed to 2 mmol/L paraquat,D.officinale formula was able to reduce ROS level and increase superoxide dismutase (SOD) activity,but the catalase activity and MDA content were not changed.Conclusion D.officinale formula was able to increase the survival rate under oxidative stress and extend the lifespan under normal conditions in C.elegans,possibly through increasing SOD activity.