红掌组织培养快繁技术研究

摘要

Taking the leaf and stem tip of Anthurium andraeanum as the experimental material ,the experi‐ment has successfully established the fast propagation system of tissue culture .The results show that when using 0 .1% HgCl2 as disinfectant ,the optimum disinfecting time is 8 minutes .The best culture medium for leaf's callus induction is MS+IAA2 .0 mg/L+6 -BA 0 .2 mg/L and that for differentiation of adventitious buds and proliferation is MS+NAA0 .5 mg/L +6-BA2 .0 mg/L .The best culture medium for adventitious bud induction and proliferation of stem tip is MS+IAA0 .2 mg/L +KT2 .0 mg/L .The best rooting medium is 1/2MS+IAA0 .5mg/L+6-BA 0 .2 mg/L .%分别采用红掌叶片和茎尖作为外植体，建立了组织培养快繁技术体系，结果表明：以0．1％ HgCl2作为消毒剂最佳的消毒时间均为8min ，叶片愈伤组织诱导、不定芽分化和增殖最佳培养基分别为 M S＋IAA2．0mg／L＋6－BA 0．2mg／L、MS＋NAA0．5mg／L ＋6－BA2．0mg／L ，茎尖不定芽诱导与增殖最佳培养基为MS＋IAA0．2mg／L ＋KT2．0mg／L ，最佳的生根培养基为1／2MS＋IAA0．5mg／L＋6－BA 0．2mg／L。