甘肃省苹果树腐烂病菌 ITS 序列分析及潜育期研究

摘要

为了明确甘肃省苹果树腐烂病菌的种类及其优势种的潜育期，通过 ITS 序列比对和离体枝条接种法对采自甘肃省不同地区的苹果树腐烂病菌进行了 ITS 序列测定、分析及潜育期研究．结果表明：甘肃省苹果树腐烂病菌有2个种，分别为Valsa mali 和Valsa malicola ，其中Valsa mali 有2个变种，分别为Valsa mali var．mali 和Valsa mali var．pyri．甘肃省苹果树腐烂病菌优势种Valsa mali 的潜育期随保湿时间增加而缩短，保湿时间为72 h时潜育期为74．3 h；温度30℃时潜育期最短，为64．3 h；潜育期随枝龄的增加而增加，嫩梢的潜育期为46．3 h；光暗交替下潜育期最短，为45．3 h．%In order to clarify incubation period and pathogen of apple treeValsa canker in Gansu Prov-ince,the ITS sequence and incubation period of pathogen were studied by analysis ITS sequence and excised twigs inoculating.The results showed that there were two species ofValsa could cause this disease in Gan-su,Valsa mali andValsa malicola .TheV.mali could be also divided into two varieties,V.mali var.mali andV.mali var.pyri.Incubation period of apple Valsa canker shortened with the increasing of moisture time.When moisture time was 72 h,the incubation period was 74.3 h.The incubation period was the shor-test at 30 ℃.The incubation period also prolonged when branch age increased,the incubation period of young shoots was 46.3 h.The incubation period under the alternation of light and darkness every 12 h was the shortest,that was 45.3 h.