放射治疗对人宫颈癌组织的增殖细胞核抗原表达的影响

摘要

AIM　To investigate changes of proliferating cell nuclear antigen (PCNA) expression following irradiation in the human cervical cancer. METHODS　Immunohistochemical staining for PCNA was performed in freezing sections of formalin-fixed cervical cancer biopsy tissue. RESULTS　Majority of cancer cells showed PCNA-immunoreac-tivity before irradiation. Following irradiation (30～40 Gy) PCNA-immunopositive staining was hardly detected in most of cancer cells. PCNA-immunoreactivity, however, increased after radiotherapy, and moderate or heavy immunopositive staining for PCNA was seen in irradiated mesenchymal tissue cells. CONCLUSION　Irradiation inhibits PCNA expression in cervical cancer cells whereas it induces the expression of PCNA in mesenchymal tissue cells. The present results suggest that expression of PCNA may be regarded as a molecular marker for evaluating the cancer cell proliferation and mesenchymal tissue repair during radiotherapy of human cervical cancer.%目的探讨放射前、后人宫颈癌组织增殖细胞核抗原（PCNA）表达的变化. 方法在多聚甲醛固定的活检癌组织冰冻切片上，进行PCNA的免疫组织化学染色. 结果放疗前的宫颈癌组织内，多数癌细胞呈PCNA免疫染色阳性；照射（30～40 Gy）后，多数癌细胞无PCNA染色，但间质细胞的PCNA免疫染色明显升高，大量间质细胞则呈中等或强阳性染色. 结论放射抑制宫颈癌细胞的PCNA表达的同时，诱导间质细胞的PCNA表达. 提示PCNA的表达状态可作为放射治疗中评价宫颈癌细胞增殖和间质组织修复的分子指标之一.