The distribution and characteristics of LDH isoenzymes in different tissues of 25spe-cies of vertebrats were comparativety analysed by discontinuous polyacrylamide gel diskelectrophoresis,combined with experiments on heat and urea inhibition.The result showed that indifferent tissues of Cienopharyngodon idellus,Carassius,ayratus,Trichiurus haumete and Miichihysmiiuy in addition to the distribution of LDH isoenzymes,composed of A and B subuits,a E4isoenzyme band,moving very fast to the positive pole,was detectedin the eye tissue of Trichiurushaumele and a F4 isoenzyme hand,moving very slow to the positive pole,was found in liver andkidney of Cienopharyngodon idellus.The A4 isoenzyme band was found in different tisseues ofPleuronichlhys cornulus,inhabiting on the bottom of the ocean.From the amphition to the mammal,with the exception of Hyta arboreu immaculata,Chinnemys reevesii,Trioyx sinensis,Gallus gallusdomeslicus and Corvus macrorhynchos,which have special electrophoretic behaviours.In
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