RFLPs IN INTERNAL TRANSCRIBED SPACERS OF RIBOSOMAL DNA FROM THREE SPECIES OF TILITIA

摘要

DNA polymorphisms were evaluated for the eight strains representing Tilletia controversa Kuhn, T. laevis Kuhn and T. tritici (Bjerk.) Winter using polymorase chain reaction and restriction fragment length polymorphism (PCR-RFLP) analysis. DNA regions coding for the internal traiscribed spacers (ITS) were amplified and digested with five four-base restriction enzymes (Msp I,Hinf I, Taq I, Hha I and Hae III). No differences were shown in the PCR-RFLP patterns among the strains tested. This indicated T, controversa, T. Iaevis and T. tritici are very closely related.