Growth hormone gene (GH) of Rhinopithecus roxellanae was amplified by PCR based on the sequences of the reported mammalian growth hormone gene for the first time. The amplified fragment was about 1.8 kb. It was cloned and its upper stream was sequenced. This sequencing region consists of a 5’flanking regulatory region, exon 1 and part of exon 11, intron 1 of growth hormone gene. Comparing the corresponding sequences of growth hormone gene between Rhinopithecus roxellanae and the porcine, we concluded that the homology reached 81% in the region, and there was high conservation in the 5’flanking sequence. The kinds of amino acids of exon 1 and exon 11 for about 90% were the same to those in pig. Many mutations occurred in the degenerate site of the triplet code. In the nucleotides of intron 1, there were only 72% homologies with those in pig. It means that introns and 3’flanking sequence maybe play an important part in growth hormone gene regulation of the different animals.
展开▼
