Characterization of a collection of Chinese fir elite genotypes using sequence.related amplified polymorphism markers

摘要

Chinese fir [Cunninghamia lanceolata(Lamb.)Hook] is a major timber tree species in China.In the present study,sequence-related amplified polymorphism(SRAP) markers were used to evaluate the polymorphisms,diversity,and relationships in a collection of Chinese fir elite genotypes(n=103) from the Lechang provenance,an endangered provenance.Thirty-five SRAP primer combinations produced 620 bands among the tested 103 Lechang provenance genotypes and 17 non-Lechang provenance genotypes;577(93.1 %) of these were polymorphic,yielding an average of 16.5 polymorphic bands per primer combination.Of the SRAP primer combinations(n=35),14 ones identified C70.0 % of the 120 genotypes.The Me12–Em19 combination had the strongest discriminatory ability with 93.3 % of the genotypes identified.An UPGMA dendrogram further showed that most of the Lechang provenance genotypes(87.4 %) grouped together(Cluster A).The Lechang genotypes had a considerable amount of genetic variation with genetic similarity from 0.40 to 0.81.The current work may facilitate the management of the endangered Lechang Chinese fir provenance.