大孔树脂分离纯化发酵液中虫草素的研究

摘要

In this study, macroporous resin was used to extract cordycepin from fermentation broth of Cordyceps militaris. By compared with the adsorption and desorption effects of six kinds of macroporous resins to cordycepin, NKA-Ⅱ macroporous resin exhibited the excellent characteristics for cordycepin purification. Through optimization of static and dynamic parameters, the time of adsorption and desorption equilibrium of cordcepin by the NKA-Ⅱ resin was 6h and 3h, respectively. The current velocity of adsorption on the column was 1 BV/h, and the eluting reagent was 70% ethanol with 4BV/h eluting rate after eliminating the impurity by 10% ethanol. By combination of those optimum conditions, the content of cordycepin, and the recovery rate achieved at 35% and 90%, respectively. Furthermore, the purity of Cordycepin was 98% through recrystallization for several times.%采用大孔树脂富集纯化北冬虫夏草发酵液中的虫草素,通过比较发现6种大孔树脂中NKA-Ⅱ型大孔树脂对虫草素的吸附与解吸效果最好.静态和动态参数优化结果表明,NKA-Ⅱ型树脂纯化虫草素的最佳吸附平衡时间为6h,解吸平衡时间为3h.优化后的动态参数为:以1 BV/h流速上样吸附,体积分数10％乙醇除杂,70％乙醇以4 BV/h的流量洗脱.该工艺所得样品虫草素质量分数达35％,纯度提高了10倍,虫草素回收率达90％以上,经反复结晶后得到纯度大于98％的虫草素.