首页> 外文期刊>渔业学报(英文) >Molecular cloning, sequencing, and expression profiles of heat shock protein 90 (HSP90) in Hyriopsis cumingii exposed to different stressors:Temperature, cadmium and Aeromonas hydrophila
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Molecular cloning, sequencing, and expression profiles of heat shock protein 90 (HSP90) in Hyriopsis cumingii exposed to different stressors:Temperature, cadmium and Aeromonas hydrophila

机译:暴露于不同胁迫下的三角帆蚌热休克蛋白90(HSP90)的分子克隆,测序和表达谱:温度,镉和嗜水气单胞菌

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摘要

Heat shock protein 90 (HSP90) represents a suite of highly conserved and multi-functional molecular chaperone proteins that play an important role in cellular stress responses.In order to better understand the expression of HSP90 in mollusks,a full-length complementary DNA (cDNA) of HSP90 (HcHSP90) was identified in Hyriopsis cumingii.HcHSP90 cDNA was 2659 bp in length,consisting of 3'and 5'-untranslated regions and an open reading frame of 2187 bp,that encoded a 728 amino acid protein.Homology analyses showed that the HcHSP90 protein was highly conserved and had 5 well-conserved family signatures of HSP90 proteins.HcHSP90 mRNA expressed in various tissues of H.cumingii.The expression level of HcHSP90 was the highest in the digestive gland.In all tissues,with the exception of the digestive gland where it was down-regulated,HcHSP90 mRNA expression was significantly induced by temperature treatments (0,5,25,and 35 C) relative to the control (15 C).Exposure ofH.cumingii to different concentrations of cadmium (50,100,and 200 μg/L),up-regulated HcHSP90 mRNA in the haemolymph and gill but without an obvious dose-dependent response.When H.cumingii were infected with Aeromonas hydrophila,HcHsP90 mRNA expression in the haemolymph was up-regulated and peaked 36 h post-infection,while in the gills it was significantly up-regulated 3 h post-infection in the gills,then remained constant until returning to pre-challenge expression levels at 36 h post-infection.The results show that HcHSP90 expression can be significantly regulated by changes in temperature,cadmium exposure and bacterial infection.We deduced that HSP90 may play an important role in helping H.cumingii to cope with environmental stress.
机译:热休克蛋白90(HSP90)代表了一组高度保守的多功能分子伴侣蛋白,它们在细胞应激反应中起重要作用。为了更好地了解HSP90在软体动物中的表达,全长互补DNA(cDNA在Hyriopsis cumingii中鉴定出HSP90(HcHSP90)。HcHSP90cDNA的长度为2659 bp,由3'和5'非翻译区组成,并具有2187 bp的开放阅读框,编码728个氨基酸。 HcHSP90蛋白是高度保守的,并具有5​​个保守的HSP90家族标记.HcHSP90 mRNA在cumingii的各种组织中表达.HcHSP90的表达水平在消化腺中最高。在所有组织中,除在温度下调的消化腺中,相对于对照(15 C),温度处理(0、5、25和35 C)显着诱导了HcHSP90 mRNA的表达。镉(50,100和200μg/ L)在血淋巴和腮中的HcHSP90 mRNA表达上调,但无明显的剂量依赖性反应。当黄瓜枯草杆菌被嗜水气单胞菌感染时,血淋巴中HcHsP90 mRNA表达升高感染后36 h的表达水平达到峰值,而在the中,感染后3 h的significantly细胞明显上调,然后保持恒定,直到感染后36 h恢复到攻击前的表达水平。 HcHSP90的表达可以通过温度,镉暴露和细菌感染的变化来显着调节。我们推断HSP90可能在帮助黄瓜枯草芽孢杆菌应对环境胁迫中起重要作用。

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  • 来源
    《渔业学报(英文)》 |2017年第002期|59-66|共8页
  • 作者单位

    Key Laboratory of Genetic Resources for Freshwater Aquaculture and Fisheries, Shanghai Ocean University, Shanghai 201306, China;

    Key Laboratory of Genetic Resources for Freshwater Aquaculture and Fisheries, Shanghai Ocean University, Shanghai 201306, China;

    Key Laboratory of Genetic Resources for Freshwater Aquaculture and Fisheries, Shanghai Ocean University, Shanghai 201306, China;

    Key Laboratory of Genetic Resources for Freshwater Aquaculture and Fisheries, Shanghai Ocean University, Shanghai 201306, China;

    Key Laboratory of Genetic Resources for Freshwater Aquaculture and Fisheries, Shanghai Ocean University, Shanghai 201306, China;

    Shanghai Engineering Research Center of Aquaculture, Shanghai 201306, China;

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